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Literature summary extracted from

  • Saha, K.; Webb, M.E.; Rigby, S.E.; Leech, H.K.; Warren, M.J.; Smith, A.G.
    Characterization of the evolutionarily conserved iron-sulfur cluster of sirohydrochlorin ferrochelatase from Arabidopsis thaliana (2012), Biochem. J., 444, 227-237.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.4 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.99.1.4 C135A the mutant shows increased specific activity compared to the wild type enzyme Arabidopsis thaliana
4.99.1.4 C199A the mutant shows reduced specific activity compared to the wild type enzyme Arabidopsis thaliana
4.99.1.4 C210A the mutant shows increased specific activity compared to the wild type enzyme Arabidopsis thaliana
4.99.1.4 C213A the mutant shows reduced specific activity compared to the wild type enzyme Arabidopsis thaliana
4.99.1.4 C219A the mutant shows increased specific activity compared to the wild type enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.99.1.4 chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.99.1.4 Fe2+ contains 1.93 mol Fe2+ per mol of protein Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.99.1.4 18600
-
 2 * 18600, estimated from SDS-PAGE Arabidopsis thaliana
4.99.1.4 36010
-
 calculated from gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.4 sirohydrochlorin + Fe2+ Arabidopsis thaliana
-
 siroheme + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.4 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.4 Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.99.1.4 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.99.1.4 33.25
-
 mutant enzyme C199A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
4.99.1.4 36.08
-
 mutant enzyme C213A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
4.99.1.4 38.5
-
 wild type enzyme, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
4.99.1.4 43
-
 mutant enzyme C219A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
4.99.1.4 44.67
-
 mutant enzyme C135A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
4.99.1.4 55.17
-
 mutant enzyme C210A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.4 sirohydrochlorin + Fe2+
-
 Arabidopsis thaliana siroheme + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
4.99.1.4 homodimer 2 * 18600, estimated from SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.99.1.4 SirB
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.99.1.4 4Fe-4S-center
-
 Arabidopsis thaliana